The Molecular Techniques Laboratory is a unit that provides access to specialized laboratory facilities and technical support for conducting research using molecular biology techniques. The Laboratory’s infrastructure is used by both researchers and doctoral students from all departments of the Institute of Systematics and Evolution of Animals Polish Academy of Sciences.
The Laboratory carries out a wide range of molecular biology analyses, including DNA and RNA extraction, quantitative and qualitative assessment of nucleic acids, DNA amplification using the polymerase chain reaction (PCR), Sanger DNA sequencing, fragment length analysis by capillary electrophoresis, DNA cloning in biological vectors, as well as the preparation of amplicon libraries and genomic DNA for next-generation sequencing (NGS).
Maciej Kociński
kocinski@isez.pan.krakow.pl
(48 12) 422-70-06 w. 43
Equipment in the Labolatory of Molecular Techniques:
ABI 3130xl sequencer
5 thermocyclers: PTC-200 MJ Research, Eppendorf Mastercycler Pro, Eppendorf Mastercycler ProS, SensoQuest Labcycler, Biometra TPersonal
7 centrifuges: Eppendorf 5804 with rotor and deepwell plate centrifuge adaptors, Eppendorf 5430 with plate centrifuge adaptor, Eppendorf 5415D and 5424 with rotor for 24 1.5-2ml tubes and centrifuges with cooling: Eppendorf 5415R and 5424R with 24 tube rotor for 1.5-2ml tubes, Eppendorf 7502R with Swing-Bucket rotor for 15 and 50ml tubes
Spectrophotometer Nanodrop ND-1000
8 electrophoresis devices on agarose gels
Gel archiving system: transilluminator connected to a digital camera and to computer
Shaker with GFL incubator
Scotsman AF100 ice machine
3 laminar chambers
Autoclave
CL-1000 Ultraviolet Crosslinker UVP
Biosan Thermomixer
2 dry heating blocks
3 water baths
Elga Purelab Flex water treatment unit 2
Eppendorf Concentrator plus
Head of the Laboratory
Dr Natalia Sawka-Gądek
sawka@isez.pan.krakow.pl
(48 12) 422-70-06 w. 43